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目的:分析丹参对冠心病的有效活性化合物及其靶点的作用机制。方法:应用中药系统药理学技术平台(TCMSP)数据库筛选出丹参中的潜在化学活性物质及相关靶点蛋白,采用基因组注释数据库平台(Genecards)预测冠心病的作用靶点,并运用uniprot数据库查询与之相对应的基因名称,借助Cytoscape(3.7.2)软件,构建"药物-疾病-靶点"的网络图可视化,通过String数据库平台构建蛋白互作网络,再利用Bioconductor平台和R语言GO富集分析和KEGG富集分析。结果:通过筛选,共获得丹参的化学物质65种,与冠心病相关的关键化学物质54种,丹参-冠心病的共同靶点50个;获得PPI核心基因为ESR1、CCND1、FOS、NCOA1、APP、NR3C1、AR、NCOA2、RELA、EGFR、IL6、NYC、PGR、PPARG、RB1、CASP3;获得73条GO生物学功能包括类固醇激素受体活性、核受体活性、转录因子活性等,和19条相关信号通路,涉及内分泌抵抗;PI3K-Akt信号通路;细胞凋亡等。结论:丹参治疗冠心病体现了中药多成分、多靶点的特点,可以通过各种途径实现对冠心病发生发展过程的调控,为后续实验提供了思路和依据。
Abstract：Objective: To analyze the active mechanism of salvia miltiorrhiza on coronary heart disease and its target mechanism. Methods: An important systematic pharmacological technology platform(TCMSP) database was used to screen potential chemically active substances and related target proteins in Salvia miltiorrhiza. The genome annotation database platform(Genecards) was used to predict the targets of coronary heart disease. The uniprot database was used to query and Corresponding gene names, with the help of Cytoscape(3.7.2) software, to build a visualization of the "drug-disease-target" network diagram, construct a protein interaction network through the String database platform, and then use the Bioconductor platform and R language GO enrichment Analysis and KEGG enrichment analysis. Results: Through screening, 65 species of salvia miltiorrhiza, 54 key chemicals related to coronary heart disease, and 50 common targets of salvia miltiorrhiza and coronary heart disease were obtained. The core genes of PPI were ESR1, CCND1, FOS, NCOA1, and APP,NR3 C1, AR, NCOA2, RELA, EGFR, IL6, NYC, PGR, PPARG, RB1, CASP3; obtained 73 GO biological functions including steroid hormone receptor activity, nuclear receptor activity, transcription factor activity, etc., and 19 Related signaling pathways involve endocrine resistance; PI3 K-Akt signaling pathway; apoptosis and so on. Conclusion: Salvia miltiorrhiza treatment of coronary heart disease reflects the characteristics of multi-component and multi-target of traditional Chinese medicine. It can regulate the occurrence and development of coronary heart disease through various channels, and provides ideas and basis for subsequent experiments.
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基本信息:
DOI：10.13210/j.cnki.jhmu.20200218.003
中图分类号:R285
引用信息:
[1]苏丙贺.基于网络药理学探讨丹参对冠心病的作用机制[J].海南医学院学报,2020,26(05):340-346.DOI:10.13210/j.cnki.jhmu.20200218.003.
基金信息:
第六批全国老中医药专家学术经验继承人项目[国中医药人教发(2017)29号]~~
2020-02-19
2020-02-19
2020-02-19