| 175 | 2 | 13 |
| 下载次数 | 被引频次 | 阅读次数 |
目的:探讨诱导血红素氧合酶-1(hemeoxygenase-1,HO-1)与氧化低密度脂蛋白(oxidizedlow density lipoprotein,ox-LDL)在动脉粥样硬化(atherosclerosis,AS)发生中的作用。方法:利用高脂饮食法建立大鼠动脉粥样硬化模型,40只Wistar大鼠随机均分为4组:对照组(A组)、单纯高脂饲料组(B组)、银杏叶提取物(extract of ginkgo biloba,EGB)组(C组)及锌原卟啉(Zinc protoporphyrinⅨ,ZnPPⅨ)组(D组),应用免疫组织化学染色法检测HO-1蛋白的表达,采用ELISA法测定大鼠血浆中oxLDL的含量。结果:与A、B、D组相比,C组HO-1蛋白阳性表达程度最强,差异具有统计学意义(P<0.05);与A、B、C组相比,D组ox-LDL蛋白的含量最高,差异具有统计学意义(P<0.05);分析动脉粥样硬化中HO-1蛋白表达与ox-LDL含量之间的关系,发现二者呈负相关(P<0.05)。结论:EGB诱导HO-1蛋白的表达抑制ox-LDL表达,而ZnPPⅨ抑制HPO-1蛋白表达促进ox-LDL表达。因此,可以选用HO-1蛋白诱导剂来下调ox-LDL蛋白的表达实现抑制动脉粥样硬化的目的。
Abstract：Objective:To explore effect of heme oxygenase-1(HO-1)and oxidized low density lipoprotein(ox-LDL)on rats with atherosclerosis(AS).Methods:A total of 40 healthy Wistar rats were divided into 5 groups randomly:control group(group A),simple high-fat diet group(group B),Ginkgo biloba group(group C),zinc protoporphyrinⅨ(ZnPPⅨ)group(group D).HO-1 expression was detected by immunohistochemical assay,the expression of HO-1 protein was detected by immunocytochemical assay.Results:HO-1 expression was highest in Group C(P<0.05).ox-LDL level was highest in Group D(P<0.05).There was negative correlation between HPO-1 and ox-LDL expression(P<0.05).Conclusions:EGB induces HO-1 expression to inhibit expression of ox-LDL,while ZnPPⅨ can inhibit HO-1 expression to promote ox-LDL expression.
1 Lund AK,LuceroJ,Lucas S,et al.Vehicular emissions induce vascular MMP-9expression and activity associated with endothelin-1-mediated pathways[J].ArteriosclerThrombVasc Biol,2009,29(4):511-517.
2 Wang X,Pu H,Ma C,et al.Adiponectin abates atherosclerosis by reducing oxidative stress[J].Med SciMonit,2014,2(20):1792-800.
3 Kuhn H,Banthiya S,van Leyen K.Mammalian lipoxygenases and their biological relevance[J].BiochimBiophys Acta,2014,11.
4 姜健,华建红.IMT、Ox-LDL与血液流变学相关指标在动脉粥样硬化诊断中的价值[J].检验医学与临床,2010,7(19):2079-2081.
5 Naito Y,Takagi T,Higashimura Y.Heme oxygenase-1 and anti-inflammatory M2 macrophages[J].Arch Biochem Biophys,2014,18.
6 Waltz P,Carchman EH,Young AC,et al.Lipopolysaccaride induces autophagic signaling in macrophages via a TLR4,heme oxygenase-1dependent pathway[J].Autophagy,2011,7(3):315-320.
7 Smith BW,King JL,Miller RJ,et al.Optimization of a Low Magnesium,Cholesterol-Containing Diet for the Development of Atherosclerosis in Rabbits[J].J Food Res,2013,2(1):168-178.
8 Ferreira PF,Zago VH,D'Alexandri FL,et al.Oxidized lowdensity lipoproteins and their antibodies:relationships with the reverse cholesterol transport and carotid atherosclerosis in adults without cardiovascular diseases[J].ClinChim Acta,2012,413(19-20):1472-1478.
9 Farrokhi E,Samani KG,Chaleshtori MH.Oxidized low-density lipoprotein and upregulated expression of osteonectin and bone sialoprotein in vascular smooth muscle cells[J].Lab Med,2014,45(4):297-301.
10 Brinkley TE,Kume N,Mitsuoka H,et al.Variation in the human lectin-like oxidized low-density lipoprotein receptor 1(LOX-1)gene is associated with plasma soluble LOX-1levels[J].Exp Physiol,2008,93(9):1085-1090.
11 Yet SF,Layne MD,Liu X,et al.Absence of heme oxygenase-1 exacerbates atherosclerotic lesion formation and vascular remodeling[J].FASEB J,2003,17(12):1759-1761.
12 Ishikawa K,Navab M,Leitinger N,et al.Induction of heme oxygenase-1inhibits the monocyte transmigration induced by mildly oxidized LDL[J].J Clin Invest,1997,100(5):1209-1216.
13 Son Y,Lee JH,Chung HT,et al.Therapeutic roles of heme oxygenase-1in metabolic diseases:curcumin and resveratrol analogues as possible inducers of heme oxygenase-1[J].Oxid Med Cell Longev,2013,2013:639541.
14 Pae HO,Jeong GS,Jeong SO,et al.Roles of heme oxygenase-1 in curcumin-induced growth inhibition in rat smooth muscle cells[J].ExpMol Med,2007,39(3):267-277.
15 Rider CV,Nyska A,Cora MC,et al.Toxicity and Carcinogenicity Studies of Ginkgo biloba Extract in Rat and Mouse:Liver,Thyroid,and Nose Are Targets[J].ToxicolPathol,2013,42(5):830-843.
16 陈临溪,王蓉蓉,黄红林,等.银杏叶提取物对饲高胆固醇兔动脉粥样硬化和低密度脂蛋白体氧化的作用[J].心肺血管病杂志,2001,20(1):46-50.
17 Jeney V,Balla G,Balla J.Red blood cell,hemoglobin and heme in the progression of atherosclerosis[J].Front Physiol,2014,2(5):379.
18 杨晓玉,汪红霞,魏宗德,等.银杏叶提取物诱导大鼠主动脉血管平滑肌细胞HO-1的表达及细胞信号通路研究[J].中国病理生理杂志,2008,24(12):2324-2327.
基本信息:
DOI：10.13210/j.cnki.jhmu.20141114.015
中图分类号:R543.5
引用信息:
[1]万冬宇,刘恒亮,张志.HO-1与ox-LDL在大鼠动脉粥样硬化发生中的作用及相关性分析[J].海南医学院学报,2015,21(02):161-163+168.DOI:10.13210/j.cnki.jhmu.20141114.015.
基金信息:
四川省自然科学基金项目(04JY029_021_3)~~
2014-11-14
2014-11-14
2014-11-14