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目的:研究杜仲和补骨脂处理对离体培养成骨细胞增殖的影响。方法:取1日龄的SD大鼠、分离培养露骨中的成骨细胞并分为4组,A组用不含药物及血清的DMEM处理,B组用杜仲终浓度为10μg/L的无血清DMEM处理,C组用补骨脂终浓度为10μmol/L的无血清DMEM处理,D组用杜仲终浓度为10μg/L及补骨脂终浓度为10μmol/L的无血清DMEM处理。处理后24小时,检测细胞中凋亡基因和增殖基因的mRNA表达量。结果:B组、C组、D组细胞中Bax、Fas、FasL、HSG的mRNA表达量均显著低于A组,c-fos、c-jun、CyclinD1、Egr-1、NDRG1的mRNA表达量均显著高于A组;D组细胞中Bax、Fas、FasL、HSG的mRNA表达量均显著低于B组和C组,c-fos、c-jun、CyclinD1、Egr-1、NDRG1的mRNA表达量均显著高于B组和C组。结论:杜仲和补骨脂能够促进成骨细胞的增殖且两药联用具有协同作用。
Abstract：Objective:To study the effect of eucommia and fructus psoraleae treatment on the proliferation of osteoblasts cultured in vitro.Methods:One-day-old SD rats were taken,the osteoblasts in exposed bone were separated,cultured and divided into 4groups,group A were treated with DMEM without drugs or serum,group B were treated with serum-free DMEM containing eucommia with final concentration 10μg/L,group C were treated with serum-free DMEM containing fructus psoraleae with final concentration 10μmol/L,and group D were treated with serum-free DMEM containing eucommia with final concentration 10μg/L and fructus psoraleae with final concentration 10μmol/L.Twenty-four hours after treatment,the mRNA expression of apoptosis genes and proliferation genes in cells were detected.Results::Bax,Fas,FasL and HSG mRNA expression in group B,group C and group D were significantly lower than those in group A while c-fos,c-jun,CyclinD1,Egr-1and NDRG1 mRNA expression were significantly higher than those in group A;Bax,Fas,FasL and HSG mRNA expression in group D were significantly lower than those in group B and group C,and c-fos,c-jun,CyclinD1,Egr-1and NDRG1 mRNA expression were significantly higher than those in group B and group C.Conclusions:Both eucommia and fructus psoraleae can promote the osteoblast proliferation,and the combination of the two drugs has synergistic effect.
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基本信息:
DOI：10.13210/j.cnki.jhmu.20170627.016
中图分类号:R285.5
引用信息:
[1]邹泽良,吴峰.杜仲补骨脂促进离体培养成骨细胞增殖的实验研究[J].海南医学院学报,2017,23(12):1593-1595+1599.DOI:10.13210/j.cnki.jhmu.20170627.016.
基金信息:
佛山市科技局项目(20150318)~~
2017-06-27
2017-06-27
2017-06-27