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目的:研究miR-186在慢性阻塞性肺疾病患者血清外泌体中的表达,探讨其与慢性阻塞性肺疾病(COPD)的临床意义。方法:收集本院53例COPD患者与53例正常人对照外周血血清,抽提外泌体并进行电镜鉴定外泌体特征,RT-PCR方法检测血清外泌体中miR-186的表达量并统计分析COPD组与正常组表达差异,分析miR-186与FEV1%预计值相关性,以及ROC曲线分析血清外泌体miR-186在COPD的诊断价值。结果:透射电镜观察到血清外泌体直径约100 nm的球形结构形态;COPD组血清外泌体中miR-186相对表达量明显高于正常对照组(t=15.47,P<0.001);miR-186表达水平与COPD患者的FEV1%预计值呈负相关(r=-0.502 4,P<0.000 1);miR-186区分COPD患者与正常人的ROC曲线下面积为0.840 9(95%置信区间:0.687 5～0.994 3)。结论:血清外泌体中miR-186高表达可能是危险因素,可能作为COPD的诊断指标。
Abstract：Objective: To study the expression of miR-186 in serum exosomes of patients with chronic obstructive pulmonary disease, and to explore its clinical significance with chronic obstructive pulmonary disease. Methods: The peripheral blood sera of 53 COPD patients and 53 normal persons in our hospital were collected. Extraction of exosomes and electron microscopy to identify the characteristics of exosomes. RT-PCR method was used to detect the miR-186 expression level in serum exosomes. And statistical analysis was conducted to compare the expression difference between the COPD group and the normal group, the correlation between miR-186 and FEV1% predicted value, and ROC curve analysis of the diagnostic value of serum exosome miR-186 in COPD. Results: The relative expression of miR-186 in serum exosomes of COPD group was 3.24±0.14, which was significantly higher than that of normal control group 1.03±0.04(t=15.47,P<0.001). The expression level of miR-186 was negatively correlated with the predicted FEV1% of COPD patients(r=-0.502 4,P<0.000 1). The area under the ROC curve between miR-186 and COPD patients was 0.840 9(95% CI: 0.687 5-0.994 3). Conclusions: The high expression of miR-186 in serum exosomes may be a risk factor and may be used as a diagnostic indicator of COPD.
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基本信息:
DOI：10.13210/j.cnki.jhmu.20200528.004
中图分类号:R563.9
引用信息:
[1]谢羽飞,赵洁,杨益秀,等.miR-186在慢性阻塞性肺疾病患者血清外泌体中表达与临床意义[J].海南医学院学报,2020,26(24):1855-1858+1864.DOI:10.13210/j.cnki.jhmu.20200528.004.
基金信息:
国家自然科学基金资助项目(81160008、81660013)~~
2020-05-28
2020-05-28
2020-05-28