海南医科大学学报

目的：探讨三七总皂苷（Panax notoginseng saponin, PNS）改善抑郁样行为小鼠的生精功能及可能机制。方法：40只C57BL/6J雄性小鼠随机分成对照组（Control组）和模型组，模型组小鼠于右后爪足底注射完全弗氏佐剂（Complete Freund's Adjuvant,CFA）诱导抑郁样行为模型；抑郁样行为小鼠模型建立后再分为模型组（CFA-D组）和药物干预组（CFA-D+PNS组）；旷场实验检测小鼠活动情况，喷糖实验和强迫游泳实验检测小鼠抑郁样行为；检测小鼠睾丸和附睾重量、睾丸系数、附睾系数及精子计数检测小鼠生殖器官及精子数量等；H&E染色观察小鼠睾丸生精细胞形态变化、精子涂片计数精子畸形率；通过ELISA检测小鼠血清睾酮（T）、黄体生成素（LH）、促性腺激素释放激素（GnRH）、卵泡刺激素（FSH）变化情况。结果：与Control组相比，模型组小鼠旷场内框活动时间（P<0.001）和活动距离（P<0.001）显著降低、舔背时间减少（P<0.001）、强迫游泳不动时间延长（P<0.01），小鼠睾丸重量（P<0.001）、附睾重量（P<0.01）、睾丸系数（P<0.001）、附睾系数（P<0.001）及精子计数（P<0.001）均降低，精子头（P<0.001）、颈（P<0.05）、尾（P<0.001）畸形率显著增高，睾丸生精上皮细胞排列松散且数量减少，同时，血清T(P<0.01)、FSH(P<0.001)、GnRH(P<0.001)、LH(P<0.001)表达明显减少；PNS干预后，小鼠内框活动时间（P<0.01）和活动距离（P<0.001）明显升高、舔背时间延长（P<0.001）、强迫游泳不动时间偏短（P<0.01），小鼠睾丸重量（P<0.001）、附睾重量（P<0.01）、睾丸系数（P<0.001）、附睾系数（P<0.05）及精子计数（P<0.01）明显升高，精子头（P<0.01）、颈（P<0.05）、尾（P<0.01）畸形率明显降低，睾丸生精上皮细胞排列紧密且数量增多，同时，血清T(P<0.05)、FSH(P<0.001)、GnRH(P<0.01)、LH(P<0.001)浓度增高。结论：PNS明显改善抑郁样行为小鼠生殖器官萎缩、生精功能障碍；同时血清中T、LH、GnRH、FSH等浓度均升高，从而改善抑郁样行为小鼠的生精功能。

Objective: To explore the effect of Panax notoginseng saponin(PNS) on spermatogenesis in mice with depressionlike behavior and its possible mechanism. Methods: A total of 40 male C57 BL/6J mice were randomly divided into the Control group and the model group(Complete Freund's Adjuvant, CFA). The depression-like behavior model was induced by injecting CFA into the sole of the right hind paw of mice in the model group. After the establishment of depression-like behavior model, the mice were divided into the model group(CFA-D group) and the drug intervention group(CFA-D+PNS group). Open field experiment was used to detect the activity of mice, sugar spray experiment and forced swimming experiment were used to detect depression-like behavior of mice. Testes and epididymis weight, testes coefficient, epididymis coefficient, and sperm count were detected to check reproductive organs and sperm count of mice. Morphological changes of testicular spermatogenic cells and sperm deformities were observed by H&E staining. The changes of serum testosterone(T), luteinizing hormone(LH), gonadotropin releasing hormone(GnRH), and follicle stimulating hormone(FSH) were detected by ELISA assay. Results: Compared to the Control group, the activity time(P<0.001) and activity distance(P<0.001) in the model group were significantly reduced, the back licking time was reduced(P<0.001), and the immobile time of forced swimming was prolonged(P<0.01). Testicular weight(P<0.001), epididymal weight(P<0.01), testicular coefficient(P<0.001), epididymal coefficient(P<0.001) and sperm count(P<0.001) were all decreased, and the malform rates of sperm head(P<0.001), neck(P<0.05) and tail(P<0.001) were significantly increased. The number of spermatogenic epithelial cells decreased and the expression of T(P<0.01), FSH(P<0.001), GnRH(P<0.001), LH(P<0.001) in serum decreased significantly. After PNS intervention, the inner frame activity time(P<0.01) and activity distance(P<0.001) were significantly increased, the back licking time was extended(P<0.001), and the immobile time of forced swimming was shorter(P<0.01). Testicular weight(P<0.001), epididymal weight(P<0.01), testicular coefficient(P<0.001), epididymal coefficient(P<0.05), and sperm count(P<0.01) were significantly increased, and the malform rates of sperm head(P<0.01), neck(P<0.05), and tail(P<0.01) were significantly decreased. The spermatogenic epithelial cells were closely arranged and increased in number. Meanwhile, serum T(P<0.05), FSH(P<0.001), GnRH(P<0.01), LH(P<0.001) were increased. Conclusion: PNS can significantly improve reproductive organ atrophy and spermatogenic dysfunction in depression-like mice. At the same time, the concentrations of T, LH, GnRH, and FSH in serum were increased, thus improving spermatogenic function of depression-like behavior mice.