海南医科大学学报

目的:本研究旨在分析呼吸机相关性肺损伤中内质网应激的参与机制及沙丁胺醇的治疗作用,为相关的临床呼吸机肺损伤治疗提供参考。方法:将80只Wistar大鼠随机分为5组:对照组、大潮气量模型组、沙丁胺醇低剂量和高剂量组。除对照组大鼠外,其余各组大鼠给予大潮气量复制肺损伤模型,低剂量、中剂量和高剂量沙丁胺醇组大鼠在通气前进行药物预灌注,模型组大鼠在通气前给予等剂量的生理盐水。Real-time PCR和免疫印迹法检测各组大鼠内质网应激相关蛋白PERK、CHOP、ATF6及BIP的表达情况。结果:模型组大鼠肺组织中内质网应激蛋白PERK、CHOP、ATF6及BIP的表达较对照组大鼠明显增强,差异具有统计学意义(P<0.05);而沙丁胺醇可以明显抑制上述蛋白的表达异常,具有明显的改善作用,差异具有统计学意义(P<0.05)。结论:沙丁胺醇可以通过抑制呼吸机相关性肺损伤肺组织中过度激活的内质网应激发挥改善作用。

Objective:To investigate the mechanism of endoplasmic reticulum stress in ventilator-related lung injury.Methods:80Wistar rats were divided into 5groups randomly including control group,model group and low,medium and high dose salbutamol group.Except the control group,all the other groups were used for model establishment.Before ventilation,salbutamol groups were intervened with drug perfusion,while the model group was intervened with equal volume of normal saline.The expression of endoplasmic reticulum stress key molecules was detecteded by western blotting and Real time PCR method.Results:Expression of PERK,CHOP,BIP and ATF6 in model group were all enhanced greatly when compared with control group(P<0.01).Salbutamol can inhibit the abnormal expression of the aforementioned parameters greatly(P<0.05).Conclusion:Salbutamol alleviates ventricular lung injury by inhibiting activated endoplasmic reticulum stress and can be taken as references for treatment in clinical work.