海南医科大学学报

目的:探讨布拉氏酵母菌联合瑞安吉口服液对轮状病毒肠炎(RVE)患儿细胞免疫功能的影响。方法:选取2016年8月~2017年7月收治的RVE患儿96例为研究对象,随机数字表法分为观察组和对照组各48例,2组患儿均给予维持水电解质及酸碱平衡、抗病毒、补液等治疗,对照组给予瑞安吉口服液,5~12个月5mL/次,2次/d;>12个月5mL/次,3次/d,观察组在对照组基础上联合布拉氏酵母菌,<12个月0.125g/次,1次/d,>12个月0.25g/次,2次/d,温开水冲服,2组均治疗1周后对比评价。2组均采集治疗前后空腹外周静脉血,采用酶联免疫吸附(ELISA)法测定血清肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-8(IL-8);流式细胞仪测定CD₃~+、CD₄~+、CD₈~+,计算CD₄~+/CD₈~+;采用免疫单扩散法检测免疫球蛋白A、G(IgA、G)。结果:观察组治疗后TNF-α、IL-6、IL-8低于对照组(P<0.05);观察组治疗后CD3+、CD₄~+、CD₄~+/CD₈~+高于对照组,CD₈~+低于对照组(P<0.05);观察组治疗后IgA、IgG高于对照组(P<0.05)。结论:布拉氏酵母菌联合瑞安口服液能够抑制RVE患儿机体炎性因子的表达,增强细胞免疫功能,有效改善临床症状。

Objective:To explore the effect of Saccharomyces boulardii(S.boulardii)in combined with Ruianji oral liquid on the cellular immune function in children with rotavirus enteritis(RVE).Methods:A total of 96 children with RVE who were admitted in our hospital from August,2016 to July,2017 were included in the study and randomized into the observation group and the control group with 48 cases in each group.The patients in the two groups were given water-electrolyte and acidbase balance maintaining,antivirus,and fluid infusion.The patients in the control group were given Ruianji oral liquid,5 mL/time from 5 months to 12 months,2 times/d;5 mL/time greater than 12 months,3 times/d.On the above basis,the patients in the observation group were given S.boulardii,0.125 g/time when less than 12 months,1 time/d;0.25 g/time when greater than 12 months,2 times/d,taking with warm water.After 1-week treatment,the efficacy was evaluate.The fasting peripheral venous blood before and after treatment in the two groups was collected.ELISA was used to detect the serum TNF-α,IL-6,and IL-8.FCM was used to detect CD₃~+,CD₄~+,and CD₈~+.CD₄~+/CD₈~+ was calculated.SRID was sued to detect IgA and IgG.Results:TNF-α,IL-6,and IL-8 after treatment in the observation group were significantly lower than those in the control group(P<0.05).CD₃~+,CD₄~+,and CD₄~+/CD₈~+ after treatment in the observation group were significantly higher than those in the control group,while CD₈~+ was significantly lower than that in the control group(P<0.05).IgA and IgG after treatment in the observation group were significantly higher than those in the control group(P<0.05).Conclusions:S.boulardii in combined with Ruianji oral liquid can inhibit the expressions of inflammatory cytokines in children with RVE,strengthen the cellular immune function,and effectively improve the clinical symptoms.