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目的：研究益肾祛痛颗粒对骨癌痛模型大鼠痛觉敏化及骨破坏的影响。方法：Walker256细胞经腹水传代后注射于雌性Wistar大鼠胫骨制备骨癌痛模型，60只大鼠随机分成假手术组、模型组及益肾祛痛颗粒低剂量组、中剂量组、高剂量组和盐酸曲马多阳性药组，造模后第14天给药，连续给药14 d，观察各组大鼠痛阈值及后肢负重差。结果：与模型组比较，益肾祛痛颗粒各组均可提高大鼠痛阈值，减小双后肢负重差（P<0.05），与阳性药组比，益肾祛痛颗粒中剂量组在提高大鼠机械性痛阈值、热痛阈值方面无统计学差异（P>0.05），益肾祛痛颗粒各组大鼠后肢负重差值均小于阳性药组（P<0.05）。结论：益肾祛痛颗粒可以缓解骨癌痛大鼠机械性痛觉敏化，并能减轻骨癌痛大鼠的骨质破坏。
Abstract：Objective:To study the effects of Yishen Qutong granula on pain sensitization and bone destruction of rats with bone cancer pain. Methods:Walker256 cells were passaged in ascites and injected into the tibia of female Wistar rats to prepare the bone cancer pain model. On the 14th day after model establishment,60 rats were randomly divided into model group,sham-operated group,Yishen Qutong granula low、middle、high dose group and tramadol hydrochloride positive control group. After continuous administration for 14 days,the mechanical pain threshold,thermal threshold and weight-bearing difference of both hind limbs were observed. Results:Compared with the model group,Yishen Qutong groups increased the mechanical pain threshold,thermal pain threshold and reduced the weight difference of both hind limbs(P<0.05). Compared with the positive drug group,there was no significant difference in increasing the mechanical pain threshold and thermal pain threshold of rats in the medium dose group of Yishen Qutong(P>0.05),and Yishen Qutong granula significantly reduced the weight-bearing difference of hind limbs in all groups(P<0.05). Conclusion:Yishen Qutong granula can relieve pain sensitization and alleviate bone damage in rats with bone cancer pain.
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基本信息:
DOI：10.13210/j.cnki.jhmu.20220705.003
中图分类号:R285.5
引用信息:
[1]宋洪丽,冯永利,王耀焓,等.益肾祛痛颗粒对骨癌痛大鼠痛觉敏化及骨破坏的影响[J].海南医学院学报,2022,28(22):1688-1693.DOI:10.13210/j.cnki.jhmu.20220705.003.
基金信息:
国家自然科学基金面上项目（81873283）; 北京市科委十病十药专项（Z171100001717017）~~
2022-07-06
2022-07-06
2022-07-06