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目的:研究PPARγ激动剂罗格列酮对大鼠心肌再灌注损伤的保护作用。方法:选择SD大鼠进行研究,随机分为假手术组、缺血再灌注损伤组、罗格列酮治疗组,检测心肌梗死面积、细胞凋亡指数、心肌功能指标以及凋亡相关分子的mRNA含量。结果:I/R组大鼠的梗死面积、凋亡指数大于Sham组,ROC组大鼠的梗死面、凋亡指数小于I/R组;I/R组大鼠血浆中CK-MB、cTnT含量高于Sham组,ROC组大鼠血浆中CK-MB、cTnT含量低于I/R组;I/R组大鼠心肌组织中β-MHC/α-MHC比例、bax和caspase-3含量高于Sham组,bcl-2和miR-208a的含量低于Sham组;ROC组大鼠心肌组织中β-MHC/α-MHC比例、bax和caspase-3含量低于I/R组,bcl-2和miR-208a的含量高于I/R组。结论:PPARγ激动剂罗格列酮对大鼠心肌再灌注损伤具有保护作用,能够缩小梗死面积、减少梗死细胞数目、调节促凋亡和抗凋亡基因的表达。
Abstract：Objective:To explore the protective effects of PPARγagonist rosiglitazone on myocardial reperfusion injury.Methods:SD rats were chosen and randomly divided into sham group,ischemia-reperfusion injury group and rosiglitazone treatment group.Then myocardial infarct size,apoptotic index,myocardial performance index and mRNA contents of apoptosis-related molecules were detected.Results:Infarct size and apoptotic index of I/R group were greater than those of Sham group,but infarct size and apoptotic index of ROC group were less than those of I/R group.CK-MB and cTnT contents in plasma of I/R group were higher than those of Sham group,and CK-MB and cTnT contents in plasma of ROC group were lower than those of I/R group.β-MHC/α-MHC proportion as well as bax and caspase-3contents in myocardial tissue of I/R group were higher than those of Sham group,and bcl-2and miR-208 acontents were lower than those of Sham group;β-MHC/α-MHC proportion as well as bax and caspase-3contents in myocardial tissue of ROC group were lower than those of I/R group,and bcl-2and miR-208 acontents were higher than those of I/R group.Conclusion:PPARγagonist rosiglitazone shows protective effect on myocardial reperfusion injury;it can reduce the infarct size,decrease the number of infarction cells and regulate the expression of pro-apoptotic and anti-apoptotic genes.
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基本信息:
DOI：10.13210/j.cnki.jhmu.20150626.004
中图分类号:R965
引用信息:
[1]袁博,王东琦,刘胜强,等.PPARγ激动剂罗格列酮对大鼠心肌再灌注损伤保护作用的实验研究[J].海南医学院学报,2015,21(08):1014-1017.DOI:10.13210/j.cnki.jhmu.20150626.004.
基金信息:
2014年安康市科技研究发展计划项目(2014AK03-11)~~
2015-06-26
2015-06-26
2015-06-26