海南医科大学学报

目的:研究牙周病患牙累及的牙根面经EDTA脱矿和富血小板血浆(PRP)单独及联合处理后对人牙周膜成纤维细胞附着、增殖的影响。方法:以因牙周病不能保留而拔除的患牙制备的牙根片为对照组,PRP和EDTA脱矿单独及联合处理患牙根片为实验组,用细胞计数法及四甲基偶氮唑盐(MTT)比色实验分析人牙周膜成纤维细胞在病变根面上的附着和增殖。结果:未处理组的根片上有少量的细胞附着;根片经PRP或EDTA脱矿单独处理能明显增加细胞的附着(P<0.05);二者联合处理促附着效果明显增强(P<0.01)。未处理组根片上生长的细胞随时间延长数量反而下降;经PRP或EDTA单独处理的根片上的细胞有少量的增殖(P>0.05);二者联合处理细胞增殖明显(P<0.05)。结论:EDTA根面脱矿和PRP能有效促进人牙周膜成纤维细胞在牙周病患牙根面上的附着和增殖。

Objective: To evaluate the effect of EDTA and platelet-rich plasma(PRP) on the attachment and proliferation of human periodontal ligament fibroblasts on diseased root surfaces in vitro.Methods: Periodontitis-affected teeth were collected and sectioned with a water-cooled diamond disc to prepare the 4mm×4mm diseased root slices.The diseased root surfaces slices were then scaled.Test slices were exposured to either EDTA(24%,pH=6.7) or PRP.The controls were treated with nothing.Double-sided adhesive tape was used to fix the root slices to the bottom of wells in a 48-well tissue culture plate.The treated and untreated root slices were then sterilized under UV light for 2 hours prior to seeding with cells.The culture were allowed to attach for 24 or 48 hours using standard incubation conditions.The attached and proliferated cells were visualized using MTT staining.Results: The untreated root surfaces demonstrated the least number of attached cells.The root surfaces treated with EDTA or PRP alone all significantly increased cell attachment(P<0.05).When PRP were added to previously EDTA-etched root surfaces,cell attachment was further enhanced(P<0.01).Diseased root surfaces untreated with EDTA or PRP did not enhance cell proliferation.cell proliferation onto diseased root surfaces treated with EDTA or PRP alone was slightly increased(P>0.05).When PRP were added to previously EDTA-etched root surfaces,cell proliferation was also enhanced.(P<0.05).Conclusion: Freshly prepared PRP or EDTA could effectively enhance the attachment and proliferation of human periodontal ligament fibroblasts onto human diseased root surfaces.