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目的:基于网络药理学探讨醒神止遗方(XSZYF)治疗小儿遗尿(nocturnal enuresis,NE)的作用机制。方法:运用TCMSP、DrugBank数据库、PubMed和CNKI获取醒神止遗方的有效活性成分和对应的靶点,在GeneCard、OMIM数据库获得NE的靶点,采用Cytoscape软件构建药物-疾病-靶点网络模型并进行分析,利用STRING数据库构建蛋白质相互作用网络(PPI),借助DAVID在线工具对关键靶点进行基因本体功能注释(GO)和东京基因组百科全书(KEGG)通路富集分析,运用Surflex对接软件对关键活性成分及关键靶点进行分子对接,验证网络分析结果。结果:获得醒神止遗方199个基因靶点,NE有2 486个基因靶点,网络分析结果表明,醒神止遗方治疗NE的关键靶点包括CHRM3、CHRM2、ADRB3等,KEGG富集主要涉及调节神经活性配体-受体相互作用、钙信号通路等。结论:本研究从网络药理学角度初步揭示了醒神止遗方治疗NE的物质基础和作用机制,为进一步治疗NE的提供方向和思路。
Abstract：Objective: To explore the mechanism of Xingshen Zhiyi Recipe(XSZYF) in the treatment of nocturnal enuresis(NE) based on network pharmacology. Methods: TCMSP, DrugBank databases, PubMed and CNKI were used to obtain the active ingredients and corresponding targets of XSZYF. NE targets were obtained from GeneCard and OMIM databases. Cytoscape software was used to construct a drug-disease-target network model. The analysis was performed. The protein interaction network(PPI) was constructed using the STRING database. The gene ontology functional annotation(GO) and the Tokyo Genomic Encyclopedia(KEGG) pathway enrichment analysis were performed on key targets using the DAVID online tool. Surflex docking software was used for the analysis. Docking of key active ingredients and key targets were used to verify the results of network analysis. Results: A total of 199 gene targets of XSZYF were obtained, and there were 2 486 gene targets of NE. Network analysis results showed that the key targets of XSZYF for treating NE include CHRM3, CHRM2, ADRB3, etc. KEGG enrichment mainly involved the regulation of neuroactive ligand-receptor interactions, calcium signaling pathways, etc. Conclusion: This study revealed the material basis and action mechanism of XSZYF in treating NE from the perspective of network pharmacology.
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基本信息:
DOI：10.13210/j.cnki.jhmu.20200418.001
中图分类号:R285
引用信息:
[1]马跃,张善云,孔涵,等.基于网络药理学探讨醒神止遗方治疗小儿遗尿的作用机制[J].海南医学院学报,2020,26(20):1560-1566+1573.DOI:10.13210/j.cnki.jhmu.20200418.001.
基金信息:
山东省中医药科技发展计划(2019-0972)~~
2020-04-20
2020-04-20
2020-04-20