海南医科大学学报

目的：观察电针“百会”、“肾俞”对AD模型小鼠认知障碍的改善作用，并探究其作用机制。方法：将24只模型小鼠随机分为电针组、西药组、模型组，每组各8只，另设8只C57BL/6J小鼠为空白组。电针组选取百会、肾俞，给予电流刺激，西药组给予盐酸多奈哌齐，每日1次，共计21 d。采用Morris水迷宫法对小鼠进行行为学测试，HE染色观察小鼠海马区神经细胞形态结构，免疫蛋白印迹法检测各组小鼠海马GSK-3β、β-catenin蛋白的表达。结果：与模型组相比，电针组、西药组逃避潜伏期明显缩短，在目标象限停留时间和跨越平台次数增加（P<0.05），且各治疗组海马神经元排列紧密完整，层次更加清晰。Western blot检测结果显示，与模型组相比，电针组、西药组小鼠海马区β-catenin蛋白表达显著上升，GSK-3β蛋白表达下降（P<0.05）。结论：电针“百会”和“肾俞”对APP/PS1小鼠的认知功能有明显的改善作用，其机理可能与Wnt/β-catenin信号途径激活有关。

Objective: To observe the effect of electroacupuncture(EA) at "Baihui"(GV20) and "Shenshu"(BL23) on cognitive impairment in AD model mice, and to explore its mechanism. Methods: A total of 24 model mice were randomly divided into EA, medication and model groups, 8 mice in each group. Another 8 C57BL/6J mice were used as the normal control. The rats in the EA group were treated with electrical stimulation at Baihui(GV 20) and Shenshu(BL 23), and those in the medication group were treated with donepezil hydrochloride, once a day for 21 days. Adopting the Maorris water maze method to detect the behavior of mice and using HE staining to observe the morphological structure of neurons in the hippocampal region of mice. Finally the expression of GSK-3β and β-catenin protein contents in the hippocampus of mice in each group was detected by Western blot. Results: Compared to the model group, the evasion latency of the electroacupuncture group and the western medicine group were significantly shorter, and the dwell time in the target quadrant and the number of crossing the plateau were increased(P<0.05), and the hippocampal neurons in each treatment group were closely arranged and complete, with a clearer hierarchy. Western blot assay results showed that the expression of β-catenin protein was significantly increased and GSK-3β protein expression was decreased in the hippocampal region of mice in the electroacupuncture and western medicine groups compared with the model group(P < 0.05).Conclusion: Ea at "Baihui"(GV20) and "Shenshu"(BL23) can significantly improve the cognitive function of APP/PS1 mice, which may be connected with the activation of Wnt/β-catenin signaling pathway.