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目的：构建一种包载抗菌肽（antimicrobial peptide,AMP）FR-13的壳聚糖（chitosan,CS）温敏水凝胶（FR-13/CS），研究其理化性质、抗菌效果及对小鼠创伤愈合的作用，为治疗战创伤引起的皮肤损伤提供新策略。方法：采用物理交联法制备FR-13/CS水凝胶，并通过扫描电子显微镜、流变学、机械性能测试及体外降解实验对其理化性质进行表征。此外，还评估了水凝胶的生物相容性和抗菌性能。在构建小鼠全层皮肤伤口模型的基础上，研究其促创面愈合效果。结果：FR-13/CS水凝胶显示出良好的温敏特性，具有均匀的微观结构、适宜的机械强度和生物降解性。体外抑菌实验表明FR-13/CS对大肠杆菌和金黄色葡萄球菌具有明显的抑菌效果。体内药效学实验表明FR-13/CS水凝胶明显促进小鼠皮肤创面愈合。安全性实验表明FR-13/CS具有良好的生物相容性。结论：FR-13/CS水凝胶具有抗菌活性和促进伤口愈合能力，展现出良好的临床应用前景。
Abstract：Objective: To prepare the antibacterial peptide FR-13 loaded with chitosan thermosensitive hydrogel(FR-13/CS), and to investigate its physicochemical properties, antibacterial activity, and wound healing effects in mice, for providing a new strategy in treating skin injuries caused by battle wounds. Methods: The FR-13/CS hydrogel was prepared using a physical crosslinking method, and its physicochemical properties were characterized by a scanning electron microscopy, rheology, mechanical testing, and in vitro degradation testing. Additionally, the biocompatibility and antibacterial effects of the FR-13/CS hydrogel were evaluated. By using a full-thickness skin wound model in mice, its effect on promoting wound healing was investigated. Results: The FR-13/CS hydrogel exhibited excellent thermosensitive properties, a uniform microstructure, suitable mechanical strength, and good biodegradability. The results of in vitro antibacterial tests demonstrated that FR-13/CS exhibited significant antibacterial activity against Escherichia coli and Staphylococcus aureus. In vivo pharmacodynamic experiments showed that the FR-13/CS hydrogel significantly promoted wound healing in mice. Safety assessments confirmed that FR-13/CS had good biocompatibility. Conclusion: The FR-13/CS hydrogel possesses antibacterial activity and promotes wound healing, demonstrating a promising potential for clinical applications.
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基本信息:
DOI：10.13210/j.cnki.jhmu.20250228.001
中图分类号:R318.08
引用信息:
[1]巴琪,姚佳欣,孟园园,等.抗菌肽FR-13温敏水凝胶用于皮肤创伤感染的研究[J].海南医科大学学报,2026,32(05):321-329.DOI:10.13210/j.cnki.jhmu.20250228.001.
基金信息:
北京市自然科学基金（7234405）~~
2025-02-28
2025-02-28
2025-02-28