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目的:研究桂枝茯苓丸联合二甲双胍对多囊卵巢综合征(PCOS)患者胰岛素抵抗相关炎症反应、氧化应激反应的影响。方法:选择2013年6月~2017年2月期间在攀枝花市妇幼保健院治疗的PCOS患者,随机分为两组,联合治疗组接受桂枝茯苓丸联合二甲双胍治疗、持续2个月,二甲双胍组接受二甲双胍治疗、持续两个月。采集血清测定胰岛素信号通路分子的表达量及炎症反应指标、氧化应激反应指标的蛋白含量。结果:联合治疗组血清中IRS-1、IRS-2、PI3K、AKT、GLUT4的mRNA表达量显著高于二甲双胍组,GSK-3β的mRNA表达量显著低于二甲双胍组;联合治疗组血清中NF-kB、TNF-α、IL-6、IL-18、MCP-1、ROS、MDA、8-OHdG的蛋白含量显著低于二甲双胍组且与IRS-1、IRS-2的mRNA表达量呈负相关,TAC、SOD的含量高于二甲双胍组且与IRS-1、IRS-2的mRNA表达量呈正相关。结论:桂枝茯苓丸联合二甲双胍能够通过减轻炎症反应及氧化应激反应来改善PCOS患者的胰岛素抵抗、增加胰岛素敏感性。
Abstract：Objective:To study the effects of Guizhi Fuling Pill combined with metformin on insulin resistance-related inflammatory response and oxidative stress response in patients with polycystic ovary syndrome(PCOS).Methods:PCOS patients who received therapy in Panzhihua Maternal and Child Care Service Centre between June 2013 and February 2013 were selected and randomly divided into two groups,combined therapy group received Guizhi Fuling Pill combined with metformin therapy for two months,and metformin group received metformin therapy for continuous two months.After serum pickup,the follicular fluid was collected to determine the expression of insulin signaling pathway molecules as well as the protein levels of inflammatory response indexes and oxidative stress response indexes.Results:Serum IRS-1,IRS-2,PI3 K,AKT and GLUT4 mRNA expression of combined therapy group were greatly higher than those of metformin group whereas GSK-3βmRNA expression was significantly lower than that of metformin group;serum NF-kB,TNF-α,IL-6,IL-18,MCP-1,ROS,MDA and 8-OHdG protein levels of combined therapy group were greatly below those of metformin group and negatively correlated with IRS-1 and IRS-2 mRNA expression whereas TAC and SOD levels were higher than those of metformin group and positively correlated with IRS-1 and IRS-2 mRNA expression.Conclusion:Guizhi Fuling Pill combined with metformin can reduce the inflammatory response and oxidative stress response to improve the insulin resistance and increase the insulin sensitivity in patients with PCOS.
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基本信息:
DOI：10.13210/j.cnki.jhmu.20171127.002
中图分类号:R711.75
引用信息:
[1]曹莉华,张红琼,赵倩.桂枝茯苓丸联合二甲双胍对PCOS患者胰岛素抵抗相关炎症反应、氧化应激反应的影响[J].海南医学院学报,2017,23(23):3211-3214+3218.DOI:10.13210/j.cnki.jhmu.20171127.002.
基金信息:
四川省卫生厅科研项目(120009)~~
2017-11-27
2017-11-27
2017-11-27