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目的:分析番茄红素对神经病理性疼痛模型大鼠脊髓组织中疼痛相关分子表达的影响。方法:取健康雌性SD大鼠30只,根据文献方法建立神经病理性疼痛模型,包括假手术组、模型对照组、模型治疗组各10只。将大鼠处死后获取脊髓L2-L6段,测定其中疼痛相关指标的血清水平、基因及蛋白表达情况。结果:假手术组大鼠的血清IL-17、HMGB-1、Aβ、Tau、C3水平低于模型对照组及模型治疗组,CGRP水平高于模型对照组及模型治疗组,模型治疗组大鼠的血清IL-17、HMGB-1、Aβ、Tau、C3水平低于模型对照组,CGRP水平高于模型对照组(P<0.05);假手术组大鼠的ERK、CREB、BDNF、NMDA、AMPA、c-fos mRNA表达量低于模型对照组及模型治疗组,模型治疗组大鼠的脊髓ERK、CREB、BDNF、NMDA、AMPA、c-fos mRNA表达量低于模型对照组大鼠(P<0.05);假手术组大鼠的TRPV1、NF-κB、NOS、GFAP、ERK、CREB蛋白表达量低于模型对照组及模型治疗组,Reg表达量高于模型对照组及模型治疗组(P<0.05),模型治疗组大鼠的TRPV1、NF-κB、NOS、GFAP、ERK、CREB蛋白表达量低于模型对照组,Reg表达量高于模型对照组(P<0.05)。结论:番茄红素可以有效减少神经病理性疼痛模型大鼠的促疼痛基因表达,有望成为日后神经病理性疼痛治疗的新手段。
Abstract：Objective:To analyze the effect of lycopene on the expression of pain-related molecules in spinal cord of model rats with neuropathic pain.Methods:A total of 30 healthy female SD rats were collected to establish neuropathic pain models according to the literatures,including 10 in sham operation group,10 in model control group and 10 in model treatment group.Rats were executed to obtain L2-L6 segment of spinal cord,and then serum levels of pain-related indicators as well as gene and protein expression in it were detected.Results:Serum IL-17,HMGB-1,Aβ,Tau and C3 levels of sham operation group were lower than those of model control group and model treatment group while CGRP level was higher than that of model control group and model treatment group,and serum IL-17,HMGB-1,Aβ,Tau and C3 levels of model treatment group were lower than those of model control group while CGRP level was higher than that of model control group(P<0.05);ERK,CREB,BDNF,NMDA,AMPA and c-fos mRNA expression levels of sham operation group were lower than those of model control group and model treatment group,and ERK,CREB,BDNF,NMDA,AMPA and c-fos mRNA expression levels of model treatment group were lower than those of model control group(P<0.05);TRPV1,NF-κB,NOS,GFAP,ERK and CREB protein expression levels of sham operation group were lower than those of model control group and model treatment group while Reg expression level was higher than that of model control group and model treatment group(P<0.05),and TRPV1,NF-κB,NOS,GFAP,ERK and CREB protein expression levels of model treatment group were lower than those of model control group while Reg expression level was higher than that of model control group(P<0.05).Conclusions:Lycopene can effectively decrease the expression of pain-promoting genes in model rats with neuropathic pain,and is expected to become new treatment for neuropathic pain in the future.
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基本信息:
DOI：10.13210/j.cnki.jhmu.20160308.009
中图分类号:R285.5;R-332
引用信息:
[1]彭冬华.番茄红素对神经病理性疼痛模型大鼠脊髓组织中疼痛相关分子表达的影响[J].海南医学院学报,2016,22(12):1192-1195+1199.DOI:10.13210/j.cnki.jhmu.20160308.009.
基金信息:
广东省卫生厅医学科研课题(A2011561)~~
2016-03-08
2016-03-08
2016-03-08