海南医科大学学报

目的:分析丹参注射液降低骨科止血带所致缺血再灌注损伤的作用机制,为临床治疗提供借鉴与参考。方法:选取我院创伤骨科择期行下肢手术患者60例,随机分为对照组和丹参注射液组,均采用两点穿刺联合腰硬麻醉,分析患者松止血带前及松止血带10、30、60min时股静脉血中血清超氧化物歧化酶(SOD)、丙二醛(MDA)及NOX2和促炎症因子白介素-6/10(IL-6/10)的表达。结果:松止血带前两组患者生命体征各相关指标无统计学差异(P>0.05),但松止血带10、30、60min后,治疗组MDA、NOX2及IL-6/10均高于对照组,而SOD水平均低于对照组(P<0.05)。结论:丹参注射液通过抑制NOX2介导的促炎症因子过表达以降低骨科止血带所致缺血再灌注损伤。

Objective:To explore the effect of Danshen injection on orthopaedic tourniquet induced ischemic reperfusion.Methods:A total of 60 cases undergoing lower limb operation were divided into 2groups,control and Danshen group.The serum MDA,SOD and the expression of NOX2 and IL6/10 was detected by western blotting.Results;The results revealed that the aforementioned parameters were in the same level before orthopaedic tourniquet release.But the serum MDA was increased and the expression of NOX as well as IL6/10 was up-regulated in control group when compared with Danshen group.At the meaning time,the serum SOD was increased in Danshen group with a significant difference when compared with control.Conclusions:Danshen injection alleviates the orthopaedic tourniquet induced ischemic reperfusion by inhibiting NOX2 mediated IL6/10 activation.