海南医科大学学报

目的:研究甲状腺癌超声血管化指数、血流指数与肿瘤恶性程度的相关关系。方法:选择在我院接受手术切除治疗的140例甲状腺结节患者作为研究对象,恶性甲状腺结节患者作为研究的恶性组、良性甲状腺结节患者作为研究的良性组。术前进行三维能量多普勒超声检查并测定血管化指数(VI)、血流指数(FI)以及血管化血流指数(VFI);术后收集肿瘤组织并测定血管新生以及细胞增殖、凋亡、侵袭相关分子的表达量。结果:恶性组肿瘤组织的VI、FI、VFI水平显著高于良性组;恶性组肿瘤组织中VEGF、MK、Ang-2、IGF-II、Bcl-2、Livin、Wip1、S100A4、TCF、β-catenin、SATB1的蛋白表达量显著高于良性组且与VI、FI、VFI水平呈正相关,CCNG2、p27的蛋白表达量显著低于良性组且与VI、FI、VFI水平呈负相关。结论:甲状腺癌的超声血管化指数、血流指数显著升高且与血管新生以及细胞增殖、凋亡、侵袭过程密切相关。

Objective:To study the correlation of ultrasound vascularization index and flow index of thyroid cancer with tumor malignancy.Methods:140 patients with thyroid nodule who accepted surgical resection in our hospital between May 2013 and June 2016 were selected as the research subjects,the patients with malignant thyroid nodule were included in the malignant group of the research and patients with benign thyroid nodule were included in the benign group of research.Three-dimensional power Doppler ultrasonography was conducted before operation to determine vascularization index(VI),flow index(FI)and vascularization flow index(VFI);tumor tissues were collected after operation to detect the expression of angiogenesis as well as cell proliferation,apoptosis and invasion-related molecules.Results:VI,FI and VFI levels of tumor tissue of malignant group were significantly higher than those of benign group;VEGF,MK,Ang-2,IGF-II,Bcl-2,Livin,Wip1,S100A4,TCF,β-catenin and SATB1 protein expression in tumor tissue of malignant group were significantly higher than those of benign group and positively correlated with VI,FI and VFI levels while CCNG2 and p27 protein expression were significantly lower than those of benign group and negatively correlated with VI,FI and VFI levels.Conclusion:Ultrasound vascularization index and flow index of thyroid cancer increase significantly and are closely related to the angiogenesis as well as cell proliferation,apoptosis and invasion.