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目的：探讨杵针治疗糖尿病周围神经病变对Kelch样ECH相关蛋白1(Keap1)/核因子相关因子2(Nrf2)/抗氧化反应元件（ARE）通路及氧化应激的影响。方法：将符合纳入标准的60例糖尿病周围神经病变患者随机分到对照组和杵针组，各30例。对照组给予常规治疗，杵针组在对照组的基础上加以杵针疗法，每周5次，共干预4周。两组治疗前后用比色法检查血清超氧化物歧化酶（superoxide dismutase,SOD）和谷胱甘肽过氧化物酶（glutathione peroxidase,GSH-Px）含量；酶联免疫吸附剂测定血清Nrf2信号通路相关因子表达水平；RT-PCR法测Keap1、Nrf2 mRNA表达。结果：与对照组比较，杵针组治疗后血清SOD、GSH-px水平明显升高，Keap1表达量减少，Nrf2表达量增加，Keap1 mRNA表达显著降低，Nrf2mRNA表达显著增加。结论：杵针可能通过调控Keap1/Nrf2/ARE信号通路使其下游抗氧化酶SOD和GSH-Px的产生增多，增强了机体抗氧化能力，从而保护和修复糖尿病周围神经病变患者受损的周围神经。
Abstract：Objective:To investigate the effect of pestle needle treatment on Nrf2 pathway diabetic peripheral neuropathy(DPN)of the relationship with oxidative stress in diabetic peripheral neuropathy. Methods:Patients with DPN who met the inclusion criteria were randomly divided into control and test groups with 30 patients in each group in a 1∶1 allocation ratio. Both groups were given basic treatment,and the pestle groupwas treated with needle pestle therapy 5 times a week for a total of 4 weeks of intervention. Serum SOD and GSH PX levels were examined by colorimetry before and after intervention;Serum Keap1/Nrf2/ARE signaling pathway related factors expression levels were measured by ELISA;Keap1 and Nrf2 mRNA expression was determined by RT-PCR. Results:Compared with the control group,SOD and GSH-Px in the test group were significantly increased,Keap1 expression was decreased,Nrf2 expression was increased,Keap1 mRNA expression was significantly decreased,and Nrf2mRNA expression was significantly increased. Conclusions:The pestle needle may enhance the body's antioxidant capacity by modulating the Keap1/Nrf2/ARE signaling pathway to enhance the production of its downstream antioxidant enzymes SOD and GSH Px,thereby protecting and repairing the damaged peripheral nerves in DPN patients.
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基本信息:
DOI：10.13210/j.cnki.jhmu.20211222.001
中图分类号:R246.1
引用信息:
[1]王芳,杨慧,廖顺琪,等.杵针对糖尿病周围神经病变Keap1/Nrf2/ARE通路及氧化应激的影响研究[J].海南医学院学报,2022,28(06):425-429.DOI:10.13210/j.cnki.jhmu.20211222.001.
基金信息:
四川省中医药管理局科学技术研究专项课题(2021MS544)~~
2021-12-23
2021-12-23
2021-12-23