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目的：探讨香烟烟雾通过激活NLRP3炎症小体诱导细胞焦亡导致大鼠睾丸损伤的作用及机制。方法：40只SPF级健康雄性SD大鼠随机分为对照组、低剂量（10支/d）吸烟组、中剂量（20支/d）吸烟组与高剂量（30支/d）吸烟组，吸烟组采用呼吸道静式染毒的方法吸入香烟烟雾8周后处死动物，检测睾丸组织病理学改变及细胞焦亡情况，同时检测大鼠睾丸组织中活性氧（ROS）水平及血浆中炎症相关因子IL-1β、IL-18、TGF-β1、MCP-1水平，RT-PCR法和Westernblot法检测大鼠睾丸细胞焦亡相关因子NLRP3、ASC、Caspase-1、GSDMD、IL-1β与IL-18 mRNA和蛋白表达水平。结果：香烟烟雾暴露8周后，大鼠睾丸组织出现出明显的病理损伤，低、中、高剂量吸烟组大鼠睾丸组织ROS水平、细胞焦亡水平和血浆中炎症相关因子IL-1β、IL-18、TGF-β1、MCP-1水平均显著高于对照组（P<0.05）。随着香烟烟雾暴露剂量的增加，大鼠睾丸组织中NLRP3、ASC、Caspase-1、GSDMD、IL-1β与IL-18 mRNA与蛋白表达水平逐渐升高。结论：香烟烟雾暴露可导致大鼠睾丸炎性损伤，其机制可能与NLRP3炎症小体激活导致的睾丸细胞焦亡有关。
Abstract：Objective: To explore the effect and mechanism of cigarette smoke-induced testicular injury in rats by activating NLRP3 inflammasome to induce pyroptosis. Methods: 40 SPF healthy male Sprague-Dawley rats were randomly divided into a control group, a low-dose smoking group(10 cigarettes/day), a medium-dose smoking group(20 cigarettes/day), and a highdose smoking group(30 cigarettes/day). The rats in the smoking group were sacrificed after 8 weeks of inhalation of cigarette smoke by respiratory static method. At the same time, the level of ROS in testicular tissue and the levels of inflammatory factors IL-1β, IL-18, TGF-β1, and MCP-1 in plasma were tested. The mRNA and protein expression levels of pyroptosis-related factors NLRP3, ASC, Caspase-1, GSDMD, IL-1β, and IL-18 were detected by RT-PCR and western blot. Results: After 8 weeks of cigarette smoke exposure, the testicular tissue of rats showed obvious pathological damage. The levels of ROS and pyroptosis in testicular tissue and the levels of IL-1β, IL-18, TGF-β1, and MCP-1 in plasma of rats in low-, medium-and high-dose smoking groups were significantly higher than those in the control group(P<0.05). With the increase of cigarette smoke exposure dose, the mRNA and protein expression levels of NLRP3, ASC, Caspase-1, GSDMD, IL-1β, and IL-18 in rat testis tissue increased gradually. Conclusion: Cigarette smoke exposure can cause testicular inflammatory injury in rats, and the mechanism may be related to pyroptosis caused by activation of NLRP3 inflammasome.
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基本信息:
DOI：10.13210/j.cnki.jhmu.20240611.001
中图分类号:R114
引用信息:
[1]王硕敏,胡一凡,燕贞,等.香烟烟雾通过激活NLRP3炎症小体导致睾丸细胞焦亡[J].海南医学院学报,2024,30(20):1557-1563.DOI:10.13210/j.cnki.jhmu.20240611.001.
基金信息:
海南省自然科学基金项目（821RC564）~~
2024-06-12
2024-06-12
2024-06-12