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目的:研究膀胱癌灌注化疗过程中负面情绪对肿瘤负荷及抗肿瘤免疫应答的影响。方法:选择2014年5月~2016年12月期间在资阳市第一人民医院接受膀胱灌注化疗的晚期膀胱癌患者,并根据HAMA量表和HAMD量表的评估结果分为不合并负面情绪的对照组、合并焦虑情绪的焦虑组、合并抑郁情绪的抑郁组、合并焦虑和抑郁情绪的焦虑抑郁组。化疗过程中,测定血清中肿瘤标志分子的含量、外周血中免疫细胞的含量以及尿液中凋亡基因的表达量。结果:焦虑组、抑郁组、焦虑抑郁组患者血清中DKK-1、DKK-3、OPN、CYFRA21-1的含量以及外周血中PD-1+CD4~+T细胞、PD-1+CD8~+T细胞、B7-H1+CD11c+DC细胞的含量显著高于对照组,尿液中DAP2IP、Fas、FasL、Caspase-8、PTEN的蛋白表达量显著低于对照组,且焦虑抑郁组患者血清中DKK-1、DKK-3、OPN、CYFRA21-1的含量以及外周血中PD-1+CD4~+T细胞、PD-1+CD8~+T细胞、B7-H1+CD11c+DC细胞的含量显著高于焦虑组、抑郁组,尿液中DAP2IP、Fas、FasL、Caspase-8、PTEN的蛋白表达量显著低于焦虑组、抑郁组。结论:膀胱癌灌注化疗过程中出现的焦虑和抑郁情绪会抑制凋亡基因表达以及抗肿瘤免疫应答并造成癌细胞增殖活力更为旺盛。
Abstract：Objective:To study the effect of negative emotion on tumor load and anti-tumor immune response in bladder cancer infusion chemotherapy.Methods:Patients with advanced bladder cancer who received bladder infusion chemotherapy in the First People’s Hospital of Ziyang between May 2014and December 2016were selected and divided into the control group without negative emotions,the anxiety group with anxiety,the depression group with depression and the anxiety depression group with both anxiety and depression according to the assessment results of HAMA scale and HAMD scale.The contents of tumor markers in serum,the contents of immune cells in peripheral blood and the expression of apoptosis genes in urine were detected during chemotherapy.Results:DKK-1,DKK-3,OPN and CYFRA21-1contents in serum as well as PD-1+CD4~+T cell,PD-1+CD8~+T cell and B7-H1+CD11c+DC cell contents in peripheral blood of anxiety group,depression group and anxiety depression group were significantly higher than those of control group while DAP2IP,Fas,FasL,Caspase-8and PTEN protein expression in urine were significantly lower than those of control group,and DKK-1,DKK-3,OPN and CYFRA21-1contents in serum as well as PD-1+CD4~+T cell,PD-1+CD8~+T cell and B7-H1+CD11c+DC cell contents in peripheral blood of anxiety depression group were significantly higher than those of anxiety group and depression group while DAP2IP,Fas,FasL,Caspase-8and PTEN protein expression in urine were significantly lower than those of anxiety group and depression group.Conclusion:The anxiety and depression in bladder cancer infusion chemotherapy can suppress the expression of apoptosis genes and the anti-tumor immune response to lead to more vigorous proliferation of cancer cells.
1 Rades D,Manig L,Janssen S,et al.Concurrent chemotherapy improves the overall survival of patients irradiated for locally recurrent bladder cancer[J].Anticancer Res,2017,37(3):1485-1488.
2 Andreassen BK,Aagnes B,Gislefoss R,et al.Incidence and survival of urothelial carcinoma of the urinary bladder in Norway 1981-2014[J].BMC Cancer,2016,16(1):799.
3 Canoui-Poitrine F,Reinald N,Laurent M,et al.Geriatric assessment findings independently associated with clinical depression in 1092older patients with cancer:the ELCAPA Cohort Study[J].Psychooncology,2016,25(1):104-111.
4 Tejido-Sánchez A,García-González L,Jiménez-Alcaide E,et al.Quality of life in patients with ileal conduit cystectomy due to bladder cancer[J].Actas Urol Esp,2014,38(2):90-95.
5 Phillips RM,Hendriks HR,Sweeney JB,et al.Efficacy,pharmacokinetic and pharmacodynamic evaluation of apaziquone in the treatment of non-muscle invasive bladder cancer[J].Expert Opin Drug Metab Toxicol,2017,13(7):783-791.
6 Sun DK,Wang L,Wang JM,et al.Serum Dickkopf-1levels as a clinical and prognostic factor in patients with bladder cancer[J].Genet Mol Res,2015 14(4):18181-18187.
7 Tsujimura N,Yamada NO,Kuranaga Y,et al.A novel role of dickkopf-related protein 3in macropinocytosis in human bladder cancer T24cells[J].Int J Mol Sci,2016,17(11):E1846.
8 Xu ST,Guo C,Ding X,et al.Role of osteopontin in the regulation of human bladder cancer proliferation and migration in T24cells[J].Mol Med Rep,2015,11(5):3701-3707.
9 Kuang LI,Song WJ,Qing HM,et al.CYFRA21-1levels could be a biomarker for bladder cancer:a meta-analysis[J].Genet Mol Res,2015,14(2):3921-3931.
10 Guo XG,Long JJ.Cytokeratin-19fragment in the diagnosis of bladder carcinoma[J].Tumour Biol,2016,37(10):14329-14330.
11 He H,Chang R,Zhang T,et al.ATM mediates DAB2IP-deficient bladder cancer cell resistance to ionizing radiation through the p38MAPK and NF-κB signaling pathway[J].Mol Med Rep,2017,16(2):1216-1222.
12 Jou YC,Tsai YS,Chen SY,et al.Loss of DAB2IP expression in human urothelial carcinoma is associated with poorer recurrence-free survival[J].Virchows Arch,2016,468(6):733-740.
13 El-Hamamsy M,Ghali RR,Saad AS,et al.FAS and FASL genetic polymorphisms impact on clinical outcome of malignant pleural mesothelioma[J].Onco Targets Ther,2016,7(9):6857-6863.
14 Makboul R,Refaiy A,Abdelkawi IF,et al.Alterations of mTOR and PTEN protein expression in schistosomal squamous cell carcinoma and urothelial carcinoma[J].Pathol Res Pract,2016,212(5):385-392.
15 Massari F,Santoni M,Ciccarese C,et al.The immunocheckpoints in modern oncology:the next 15years[J].Expert Opin Biol Ther,2015,15(7):917-21.
16 Jó'z wicki W,Bro.z yna AA,Siekiera J,et al.Changes in immunogenicity during the development of urinary bladder cancer:apreliminary study[J].Int J Mol Sci,2016,17(3):285.
17 Amaya CN,Wians FH Jr,Bryan BA,et al.Enhanced expression of Programmed cell death 1(PD-1)protein in benign vascular anomalies[J].Pathology,2017,49(3):292-296.
18 Bellmunt J,Powles T,Vogelzang NJ.A review on the evolution of PD-1/PD-L1immunotherapy for bladder cancer:The future is now[J].Cancer Treat Rev,2017,54:58-67.
19 Budczies J,Bockmayr M,Denkert C,et al.Pan-cancer analysis of copy number changes in programmed death-ligand 1(PD-L1,CD274)-associations with gene expression,mutational load,and survival[J].Genes Chromosomes Cancer,2016,55(8):626-639.
基本信息:
DOI：10.13210/j.cnki.jhmu.20170920.006
中图分类号:R737.14
引用信息:
[1]汪遵君.膀胱癌灌注化疗过程中负面情绪对肿瘤负荷及抗肿瘤免疫应答的影响[J].海南医学院学报,2017,23(18):2585-2588.DOI:10.13210/j.cnki.jhmu.20170920.006.
基金信息:
2005年四川省科技攻关计划基金项目(2005sz0937)~~
2017-09-20
2017-09-20
2017-09-20