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目的:研究甲状腺良性结节和恶性结节的超声造影特征比较及其与癌细胞生物学行为的相关性。方法:选择在我院手术切除的甲状腺结节患者并根据术后病理分为恶性组和良性组。术前进行超声造影检查并测定峰值强度(PI)、达峰时间(TTP)、平均通过时间(MTT);术后收集结节病灶并测定血管新生分子、增殖分子、凋亡分子的表达量。结果:恶性组患者甲状腺结节病灶PI的水平高于对照组,TTP、MTT的水平低于对照组;恶性组患者甲状腺结节病灶内VEGF、Gal-3、vWF、COX-2、BRAF、MCM7、XIAP、Survivin的mRNA表达量显著高于对照组且与PI的水平呈正相关,与TTP、MTT的水平呈负相关,RASSF1A、CCNG2、PDCD4、P53、PTEN的mRNA表达量显著低于对照组且与PI的水平呈负相关,与TTP、MTT的水平呈正相关。结论:甲状腺恶性结节的超声造影特征为血供丰富且血流阻力较小,上述特征与血管新生、细胞增殖密切相关。
Abstract：Objective:To study the contrast-enhanced ultrasound features of benign nodules and malignant nodules as well as their correlation with the biological behavior of cancer cells.Methods:Patients with thyroid nodules who received surgical resection in our hospital between April 2015 and February 2017 were selected and divided into malignant group and benign group according to postoperative pathology.Preoperative ultrasonography was performed to determine peak intensity(PI),time to peak(TTP)and mean transit time(MTT);postoperative nodule lesions were collected to detect the expression of angiogenesis molecules,proliferation molecules and apoptosis molecules.Results:PI level of thyroid nodule lesion of malignant group was higher than that of control group while TTP and MTT levels were lower than those of control group;VEGF,Gal-3,vWF,COX-2,BRAF,MCM7,XIAP and Survivin mRNA expression in thyroid nodule lesion of malignant group were significantly higher than those of control group,positively correlated with PI level,and negatively correlated with TTP and MTT levels while RASSF1 A,CCNG2,PDCD4,P53 and PTEN mRNA expression were significantly lower than those of control group,negatively correlated with PI level,and positively correlated with TTP and MTT levels.Conclusion:The contrast-enhanced ultrasound features of malignant thyroid nodules are rich blood supply and small blood flow resistance,and they are closely related to angiogenesis and cell proliferation.
1 Girardi FM.Thyroid Carcinoma Pattern Presentation According to Age[J].Int Arch Otorhinolaryngol,2017,21(1):38-41.
2 Zhang H,Yang L,Teng X,et al.The chemokine receptor CXCR7is a critical regulator for the tumorigenesis and development of papillary thyroid carcinoma by inducing angiogenesis in vitro and in vivo[J].Tumour Biol,2016,37(2):2415-2423.
3 Pessina P,Castillo VA,César D,et al.Proliferation,angiogenesis and differentiation related markers in compact and follicular-compact thyroid carcinomas in dogs[J].Open Vet J,2016,6(3):247-254.
4 Yuan Z,Quan J,Yunxiao Z,et al.Association between realtime contrast-enhanced ultrasound characteristics and thyroid carcinoma size[J].Mol Clin Oncol,2015,3(4):743-746.
5 Hong YR,Luo ZY,Mo GQ,et al.Role of Contrast-Enhanced Ultrasound in the Pre-operative Diagnosis of Cervical Lymph Node Metastasis in Patients with Papillary Thyroid Carcinoma[J].Ultrasound Med Biol,2017,43(11):2567-2575.
6 Liu Y,Liu H,Qian CL,et al.Utility of quantitative contrastenhanced ultrasound for the prediction of extracapsular extension in papillary thyroid carcinoma[J].Sci Rep,2017,7(1):1472.
7 Skuletic V,Radosavljevic GD,Pantic J,et al.Angiogenic and lymphangiogenic profiles in histological variants of papillary thyroid carcinoma[J].Pol Arch Intern Med,2017,127(6):429-437.
8 Lv Y,Sun Y,Shi T,et al.Pigment epithelium-derived factor has a role in the progression of papillary thyroid carcinoma by affecting the HIF1α-VEGF signaling pathway[J].Oncol Lett,2016,12(6):5217-5222.
9 Rago T,Scutari M,Loiacono V,et al.Low Elasticity of Thyroid Nodules on Ultrasound Elastography Is Correlated with Malignancy,Degree of Fibrosis,and High Expression of Galectin-3and Fibronectin-1[J].Thyroid,2017,27(1):103-110.
10 Al-Sharaky DR,Younes SF.Sensitivity and Specificity of Galectin-3and Glypican-3in Follicular-Patterned and Other Thyroid Neoplasms[J].J Clin Diagn Res,2016,10(3):6-10.
11 Perisa MM,Sarcevic B,Troselj KG,et al.Expression of nm23-H1and COX-2in thyroid papillary carcinoma and microcarcinoma[J].Oncol Lett,2017,13(5):3547-3555.
12 Li Q,Yuan J,Wang Y,et al.Association between the BRAF V600E mutation and ultrasound features of the thyroid in thyroidpapillary carcinoma[J].Oncol Lett,2017,14(2):1439-1444.
13 Werner TA,Dizdar L,Nolten I,et al.Survivin and XIAPtwo potential biological targets in follicular thyroid carcinoma[J].Sci Rep,2017,7(1):11383.
14 Shou F,Xu F,Li G,et al.RASSF1Apromoter methylation is associated with increased risk of thyroid cancer:a meta-analysis[J].Onco Targets Ther,2017,9(10):247-257.
15 李伟娟,刘阁玲,俞芳,等.细胞周期蛋白G2在甲状腺癌组织中表达及其对癌细胞增殖与凋亡的影响[J].中国肿瘤生物治疗杂志,2014,21(6):669-674.
16 Chen X,Wu W,Chen X,et al.Roles of phosphatidylinositol 3-kinase regulatory subunit alpha,activator protein-1,and programmed cell death 4in diagnosis of papillary thyroid carcinoma[J].Tumour Biol,2016,37(5):6519-6526.
17 Zhao J,Chi J,Gao M,et al.Loss of PTEN Expression Is Associated With High MicroRNA 24Level and Poor Prognosis in Patients With Tongue Squamous Cell Carcinoma[J].J Oral Maxillofac Surg,2017,75(7):1449.e1-1449.e8.
基本信息:
DOI：10.13210/j.cnki.jhmu.20171026.002
中图分类号:R445.1;R581;R736.1
引用信息:
[1]陈薇.甲状腺良性结节和恶性结节的超声造影特征比较及其与癌细胞生物学行为的相关性[J].海南医学院学报,2017,23(21):2983-2986.DOI:10.13210/j.cnki.jhmu.20171026.002.
基金信息:
国家中医药管理局项目基金资助编号:[2012]228~~
2017-10-26
2017-10-26
2017-10-26