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目的:研究交泰丸对糖尿病脑病小鼠认知功能的影响。方法:将db/db小鼠随机分为模型组、交泰丸高、中、低剂量组、二甲双胍组;db/m小鼠作为空白组,连续8周灌胃给药1次/d。水迷宫检测学习记忆功能;口服葡萄糖耐量实验及胰岛素耐量实验检测血糖变化及胰岛素敏感性;脑组织匀浆检测MDA含量及SOD活力;尼氏染色观察小鼠海马区神经元组织形态。结果:交泰丸可使糖尿病脑病模型小鼠的游泳探索距离和逃避潜伏期明显缩短;同时可调节血糖水平,改善胰岛素敏感性;使小鼠脑组织M D A含量降低的同时SOD活力升高;对小鼠海马组织神经元的组织形态发挥改善作用。结论:交泰丸可能通过改善小鼠糖代谢水平,抑制体内氧化应激,减轻海马病理改变,从而提升糖尿病脑病小鼠学习与认知能力,发挥一定的糖尿病脑病防治功效。
Abstract：Objective:To observe the effect of Jiao-tai-wan(JTW) on cognitive function in mice with diabetic encephalopathy.Methods:The db/db mice were randomized into the model group,the high-,medium-,and low-dose groups of JTW,and the metformin group;the db/m mice were set as the blank group,given the drug by stomach gavage once a day for eight consecutive weeks.Water maze test was conducted to detect learning memory function;oral glucose tolerance test and insulin tolerance tests were conducted to detect changes in blood glucose and insulin sensitivity;brain tissue homogenate was prepared to detect the content of MDA and the activity of SOD;and Nissl staining was applied to observe the morphology of neuronal cells in the hippocampal tissue area of the brain of the mice.Results:JTW could significantly shorten the swimming exploration distance and evasion latency of diabetic encephalopathy modeled mice,meanwhile,it could regulate the blood glucose level and improve the insulin sensitivity;it made the MDA content of mouse brain tissue decreased while SOD activity increased;it exerted an improving effect on the histomorphology of neurons in mouse hippocampal tissue.Conclusion:JTW may improve glucose metabolism,inhibit oxidative stress,and reduce hippocampal pathological changes in mice,thus enhancing learning and cognitive abilities of mice with diabetic encephalopathy,and exerting certain efficacy in preventing and controlling diabetic encephalopathy.
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基本信息:
DOI：10.13210/j.cnki.jhmu.20250103.003
中图分类号:R285.5
引用信息:
[1]苏妙芳,梁振钰,陈桂敏,等.交泰丸对糖尿病脑病小鼠认知功能的影响[J].海南医科大学学报,2025,31(19):1488-1493.DOI:10.13210/j.cnki.jhmu.20250103.003.
基金信息:
国家自然科学基金资助项目（82004246）; 海南省自然科学基金资助项目（822MS069）~~
2025-01-06
2025-01-06
2025-01-06