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目的:分别观察大鼠治疗结束及结束30 d后瞬时外向钾离子通道相关蛋白的表达情况,探讨针刺结合蹊径通脉汤对心肌梗死大鼠的持续疗效及其作用机制。方法:130只雄性SD大鼠,随机抽取20只作为对照组,其余通过高脂喂养后注射异丙肾上腺素的方法建立心肌梗死大鼠模型,根据心电图确定80只大鼠造模成功,将造模成功的大鼠随机分为模型组、针药结合组、针刺组、西药组,20只/组,并分别于治疗结束及结束30 d后处死取样。ELISA法测定人碱性成纤维细胞生长因子(bFGF)蛋白含量。Western Blot法检测大鼠Kv1.4、Kv4.2、Kv4.3、KChIP2蛋白表达量。结果:治疗结束时,相比模型组各治疗组Kv1.4、Kv4.2、Kv4.3、KChIP2及bFGF蛋白含量升高(P<0.05),其中针药结合组升高最为明显(P<0.05)。治疗结束30 d后,相比模型组各治疗组Kv1.4、Kv4.2、Kv4.3、KChIP2及bFGF蛋白含量升高(P<0.05),其中针药结合组升高最为明显(P<0.05)。相比治疗结束时的各治疗组,治疗结束30 d后的各治疗组Kv1.4、Kv4.2、Kv4.3、KChIP2及bFGF蛋白表达仍高于此时的模型组(P<0.05),其中针药结合组的效果最明显(P<0.05)。结论:针刺结合蹊径通脉汤对心肌梗死大鼠具有良好的疗效,其作用机制可能是通过瞬时外向钾离子通道,持续上调Kv1.4、Kv4.2、Kv4.3、KChIP2及bFGF蛋白含量实现。
Abstract：Objective:To determine the sustained expression of transient outward potassium channel related proteins at the end of the treatment and 30 days after the end of the treatment in rats,and to explore the sustained curative effect and mechanism of acupuncture combined with Xijing Tongmai decoction in rats with myocardial infarction.Methods:A total of 130 male SD rats were used with 20 rats randomly selected as the control group. The rest of rats were fed a high-fat diet and then injected with isoproterenol to establish the rat model of myocardial infarction. According to ECG,80 rat models were successfully established and randomly divided into model group,acupuncture + Chinese medicine group,acupuncture group and Western medicine group,with 20 rats per group. The content of bFGF protein was measured by ELISA. The protein expressions of Kv1.4,Kv4.2,Kv4.3 and KChIP2 were measured by Western blotting.Results:At the end of treatment and 30 days after the end of treatment,compared with the model group,the protein expressions of Kv1.4,Kv4.2,Kv4.3,KChIP2 and bFGF were increased in treatment groups,with the acupuncture + Chinese medicine group showing the most significant increase(P< 0.05). Compared with the treatment group at the end of treatment,the expressions of Kv1.4,Kv4.2,Kv4.3,KChIP2 and bFGF protein in treatment groups were still higher than those of the model group 30 days after the end of treatment(P< 0.05). The acupuncture + Chinese medicine group showed the better improvement than other groups(P<0.05).Conclusion:Acupuncture combined with Xijing Tongmai decoction has a sustained therapeutic effect,which may be achieved by continuously increasing the protein expressions of Kv1.4,Kv4.2,Kv4.3,KChIP2 and bFGF through transient outward potassium channel.
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基本信息:
DOI：10.13210/j.cnki.jhmu.20200922.006
中图分类号:R245
引用信息:
[1]邸静鑫,王颖,金颂峰.针刺结合蹊径通脉汤对心肌梗死大鼠瞬时外向钾离子通道相关蛋白持续表达的研究[J].海南医学院学报,2021,27(05):327-332.DOI:10.13210/j.cnki.jhmu.20200922.006.
基金信息:
辽宁省科学技术基金计划重点项目（20180530079）~~
2020-09-22
2020-09-22
2020-09-22